Described as "arguably both literally and metaphorically the most-travelled wheelchair in history", the specially-adapted chair had originally been expected to fetch just £15,000.
Proceeds from the sale of the red leather wheelchair will go to the Stephen Hawking Foundation and the Motor Neurone Disease Association. Rehana Popal, an immigration specialist in London, said that in two years she had lost six instructions on similar grounds.
Personal items of renowned physicist Stephen Hawking, including his personalized wheelchair, doctoral thesis and medals, have been auctioned for over a million dollars.
A signed copy of his 117-page dissertation - one of only five - titled Properties of Expanding Universes from 1965 secured the top price of £584,750.
One of the theoretical physicist's wheelchairs was sold at auction smashing its estimate.
A total of 22 items were sold during the online auction.More news: FM: Turkey has not revealed all the evidence on Khashoggi’s death
Hawking, who was also a cosmologist, astronomer, mathematician and a prolific author, died in March at age 76.
As previously reported, the stroller, and many other things scientific figure, was presented in the form of lots on the popular auction Christie's.
There was also a collection of his medals and awards available to prospective buyers, and they went for £296,750.
"The results of this remarkable sale, with more than 400 registered bidders from 30 different countries, demonstrate the enormous admiration and affection with which Stephen Hawking was viewed around the world", according to a statement from Thomas Venning, head of books and manuscripts for Christie's, and James Hyslop, head of science and natural history.
Sophie Hopkins, specialist in manuscripts and archives at Christie's, said much of the collection was "incredibly iconic".
"We are also giving admirers of his work the chance to acquire a memento of our father s extraordinary life in the shape of a small selection of evocative and fascinating items".