France's World Cup-winning captain Hugo Lloris avoided jail but received a 20-month driving ban and a £50,000 ($65,000) fine on Wednesday for drink-driving in central London.
Asked whether Lloris, who was found to be twice the legal limit and had a passenger in the vehicle, would remain captain, Pochettino said: "Of course".
"And of course now it is a massive lesson for him, a life lesson".
"Liverpool are one of the best teams in Europe, not only in England and have very good players, great manager and of course it will be a very tough game and will demand a lot of energy".
After initially being charged at the end of August, Pochettino stood by his goalkeeper and allowed him to wear the captain's armband in the 3-0 victory over Manchester United at Old Trafford.More news: Djokovic dismisses Del Potro to win US Open
"Of course, any woman, man, person can make a mistake".
When sentencing, the magistrate said the fine represented the seriousness of the offence as well as the goalkeeper's financial means. We need to try (to see) he understands he made a mistake. "Because he is clever he'll learn and now he is going to move on".
"We know Harry Kane very well and have worked with him for more than four years and we know it's not a situation that worries us".
And Pochettino is under no illusions over how tough the match will be for his Tottenham side.