Using a state-by-state analysis, it argues that Trump is risking a global trade war that will hit the wallets of USA consumers.
Trump's Republicans are fighting to retain control of Congress in November midterm elections.
He points to Ontario as a province that could see bigger issues, especially if the auto sector is hit hard.
"We should seek free and fair trade, but this is just not the way to do it".
The prospect of a deepening trade war has unsettled financial markets - which were further rattled on Monday by worries about the stability of the German government and the health of the Chinese economy. Farmers will pay dearly for 25 percent retaliatory tariffs on soybeans, which China plans to impose on Friday.
According to the Reuters news agency, the US Chamber of Commerce declared that his efforts to counter trade imbalances would prove counterproductive as they would damage the spending power of businesses and consumers alike.
Democratic voters will support Republicans because of ICE criticism Coney Barrett, Kavanaugh among candidates who met with Trump: report Trump administration recommends against allowing China Mobile access to United States market MORE touted his economic agenda on Tuesday and suggested that the USA economy could see further improvement once he is done "fixing" a series of global trade agreements.
In a letter to USA authorities, the European Commission, which handles trade policy for the bloc's 28 members, painted a stark picture of what the U.S. economy would face if Trump followed through on his threat.More news: Maryland shooting suspect barricaded door in 'tactical approach'
Mr. Trump's administration wants Germany - which would be hardest hit by automotive tariffs - to pressure the European Union to come to an agreement. But mounting trade tensions have opened a rift with the president.
Trump railed against his trading partners during the meeting, according to sources, and withdrew his support for a joint communique after leaving the summit, angering and bewildering some of Washington's closest allies. Retaliatory tariffs would cost billions and kill thousands of jobs.
Assuming counter-measures along the lines of those taken in response to existing United States import tariffs on steel and aluminium, up to US$294 billion of USA exports - 19% of overall U.S. exports - could be affected, the submission said.
Harley-Davidson, which dominates the heavyweight United States motorcycle market, subsequently announced it would shift some USA production overseas to avoid higher costs for European Union customers.
Owing in part to President Donald Trump's tax reform, which cut the corporate tax rate to 21% from 35%, companies listed in the S&P 500 Index boosted stock buybacks, setting a new record for the group during the first quarter.
The submission said European vehicle companies are "well established" in the United States.
Canada has struck back at US steel and aluminum tariffs by imposing punitive measures on $16.6 billion worth of American goods until Washington relents.
Global equities fell on Monday as investors anxious about an escalation of the trade disputes.
US President Donald Trump boards Air Force One prior to departure from Morristown Municipal Airport in Morristown, New Jersey, July 1, 2018, following a weekend in Bedminster, New Jersey.