The eyes of the nation will be focused on the Volgograd Arena when Southgate's exciting young side take to the field in their first major tournament match since the humiliating Euro 2016 exit to Iceland.
Players from both teams could be seen trying to wave the swarming bugs - all of which are said to be harmless - away during warm-ups for their Group G opener, with England players using insect repellent to help keep them away.
Friendly wins over Nigeria and Costa Rica did no harm to the mood nor did the fact that, unlike so many times in the past, there is little, if any, controversy surrounding the squad.
Tunisia will play without star Youssef Msakni, who suffered a torn anterior cruciate ligament on April 7.More news: Trump announces plans for Pentagon to create 'Space Force'
However, missed chances from Jesse Lingard, who was guilty of wasting a number of opportunities, and Dele Alli meant that England's lead remained slender.
Tunisia, playing its fifth World Cup has never got beyond the group stages.
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Tunisia are ranked 21st in the world. That's part of England's ideal record against African teams at the World Cup - three wins and three draws with five clean sheets.