Asked about allegations from the White House that the Eagles chose to "abandon" their fans and not attend the ceremony as a complete team, Pederson responded: "What you've seen and what you've heard is enough, and I'm not discussing it".
The President also noted that numerous Eagles weren't planning to attend the White House ceremony. "It wasn't discussed once in our meetings about the visit", Eagles defensive end Chris Long said on Twitter.
No Eagles players knelt during the anthem last season.
Trump sang along to "The Star-Spangled Banner" and "God Bless America" during a brief ceremony that included the U.S. Marine Band and the U.S. Army chorus.More news: PM Narendra Modi praises Afghanistan President Ashraf Ghani's bold initiatives towards peace
"You're going to argue with me about not wanting to go for simply a photo op with someone who I don't feel respects us as a leader".
Trump abruptly canceled the event on Monday night, citing a disagreement between him and some of the players regarding protests during the national anthem, which are meant to shine a light on police brutality and oppression of people of color. The NFL Network reported that fewer than 10 players were expected to attend.
While reporting the story, Fox News appeared to imply that Philadelphia Eagles players had been kneeling during the anthem throughout the year.
NFL owners last month approved a new anthem policy which "requires players and league personnel on the sideline to stand but gives them the option to remain in the locker room if they don't want to stand" according to the league's website.
The White House event will be held at 3 p.m. Tuesday.