The Serbia global limped out of the 4-2 win over Watford on Tuesday and Mourinho was anxious the midfielder had picked up an "important" injury.
With United nearly certain to progress in the Champions League against CSKA Moscow on Tuesday, Mourinho added: 'I think I will always try to play the best possible team. I hope he can.
The manager is remaining hopeful, but up to the point of his press conference, he could not guarantee that Matic would be fit enough to play.
Jones picked up a calf problem on worldwide duty for England against Germany, while Bailly is still suffering from a "knock".
And Mourinho subsequently hit out at England's treatment of the player, specifically that Jones had been given six painkilling injections in order to feature in a friendly.
We know that the media will try and twist any comment by Mourinho or Wenger to fuel the sensationalist headlines, but Le Prof did a good job there of giving them absolutely nothing to go on.
Southgate added: "We have got ongoing dialogue over this situation". Man United is a strong team and I expect us just to cope with the problems they will give us.More news: Police 'seek charging advice' over Bristol nightclub incident
Jose Mourinho has compared his latest clash with Arsene Wenger to the tennis rivalry between Bjorn Borg against John McEnroe.
The weekend's most high profile Premier League match, Manchester United at Arsenal, will be missing a few key players.
"Yesterday I was watching an interesting movie, Borg vs McEnroe and Borg coach was telling him that all the time, one point at a time, think about just one point and I tell my players just think about the game". It doesn't matter what comes after or what happens before.
"It is just about the game against Arsenal". So now it is Arsenal. We don't think about how many points behind we are.
Asked whether he sees himself as McEnroe and Wenger as Borg, Mourinho replied: 'I love tennis, I don't play tennis'. "I don't play tennis".
"I haven't seen them a lot and it is not down to me to judge the way they play".