Seth and Alexi, a lawyer, are already parents to son Ashe, born in 2016.
"I want to take this opportunity now, you guys obviously know, my wife and I are expecting a second baby", the late-night host said.
Meyers invited his parents Hillary and Larry, along with his brother Josh, to the Thanksgiving edition of Late Night With Seth Meyers to make the big reveal. When she asked why he was crying, he said, "Because for over a month, I've had to pretend I don't care what sex it is and I do care. I want a little boy." she recalled.
A source confirmed to Page Six that Meyers' is getting his wish as the couple of four years are expecting a boy.More news: Thanksgiving, Black Friday online sales soar to record highs
The "Late Night" host will join the likes of Ricky Gervais, Amy Poehler, Tina Fey and Jimmy Fallon, who have all emceed the awards show. Although the late-night host typically holds back from commenting on his marriage, he did share a sweet memory back in 2014 of Ashe trying on her wedding gown.
"With his natural comedic wit and innate ability to charm audiences, Seth will help us carry on the celebratory tradition of recognizing the best in television and film at the Party of the Year", Meher Tatna, president of the Hollywood Foreign Press Association, said in a statement.
Seth Meyers announces on Thursday's episode of his show that he's expecting a second baby with wife, Alexi.
The 75th edition of the award show will take place on January 7 at the Beverly Hilton in Beverly Hills.
NBC is airing a two-hour Golden Globes 75th Anniversary Special on December 13 with Will & Grace stars Eric McCormack and Debra Messing leading the proceedings.