Freeland argued that Boeing is "manipulating the United States trade remedy system to prevent Bombardier's new aircraft, the C Series, from entering the U.S. market, despite Boeing's admission that it does not compete with the C Series".
The U.S. says Montreal-based Bombardier used unfair government subsidies to sell jets at artificially low prices in the United States.
The 80% anti-dumping duty handed down by the US regulator is on top of a 219% countervailing duty which has already been handed down, a series of devastating duties for the CSeries manufacturer - amounts which will all but certainly disallow Bombardier's airplanes into the USA market.
"We will.do everything in our power to stand up for American companies and their workers", Ross said in a statement.
The Commerce Department proposed a 79.82 percent antidumping duty on Friday, on top of a 219.63 percent duty for subsidies announced last week.
Commerce said it based Bombardier's preliminary dumping margin on adverse facts available (AFA), because Bombardier failed to provide the information it requested.
She added that the Government will "vigorously defend the interests of the Canadian aerospace industry and our aerospace workers against irresponsible and protectionist trade measures".
The row has called into question the DUP-Conservative deal at Westminster, but it has also cast doubt on the British government's ability to successfully agree a trade deal with the US post-Brexit.More news: Padmavati trailer: An epic you really don't want to miss!
"Bombardier always has the option of coming into full compliance with trade laws", Boeing added.
"Following the initial determination, there is then a further call for evidence. we look to the U.S.to make sure that this is a rigorous process and is not politically influenced", Clark told lawmakers.
He said workers will fight even harder to get the duty reversed. The investigation was launched following the filing of a petition by Boeing in April, alleging one dumping margin.
The wings of Bombardier's C-Series are manufactured in Belfast at the former Shorts plant, where Bombardier now employs over 4,000 people.
Bombardier has repeatedly stressed that Americans will be hurt by the tariffs because more than half the content on the 100- to 150-seat CSeries is sourced by US suppliers, including Pratt & Whitney engines.
In a statement on Friday, she said the Canadian government was "extremely disappointed by and in complete disagreement with" the new tariff that "put [s] at risk the nearly 23,000 U.S.jobs that depends on Bombardier and its suppliers".
Canada has threatened to cancel the planned purchase of 18 Super Hornets to temporarily augment Canada's aging fleet of CF-18s.