Real Madrid, meanwhile, will also be disappointed that another injury has hampered their No. 11.
This is the latest in an increasingly long line of injury disappointments for Bale, without whom Wales have not won a game since 2013.
Sheffield United's David Brooks could be in line to make his senior debut for Wales during Friday's World Cup qualifier against Georgia after Gareth Bale, the undisputed star of Chris Coleman's squad, was ruled-out of the match through injury.
"He could have played with us (against Espanyol) but I preferred to leave him out as he had a small complaint", said Zidane on Sunday.
Bale has scored four of Wales' 12 goals so far in qualifying but has often been battling through injuries when it has come to worldwide games over the past 12 months.More news: USA to ask Cuba to cut embassy staff by 60 percent
Liverpool teenager Woodburn was named young player of the year after his dramatic World Cup introduction last month.
The 28-year-old has scored 26 goals in his 68 Wales appearances and played a huge role in their memorable run to the Euro 2016 semi-finals last summer.
His absence could mean a first worldwide start for Woodburn, whose goal off the bench against Austria in September catapulted him onto the big stage.
However, with Bale out it is unlikely that Coleman would bring in two new wingers alongside Aaron Ramsey.
"When they lose a player like that, they'll just want to win even more and show people that they don't just need him in the team to win games".